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Production of Some Virulence Factors Under Different Growth Conditions and Antibiotic Susceptibility Pattern of Aeromonas Hydrophila

By Animal Research International, University Of Nigeria, Nsukka.

Summary

Virulence is an important property of microorganisms in relation to their pathogenicity and is defined as the capacity of the organism to invade tissues, multiply and produce toxic effects.
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Abstract

ABSTRACT The production of some virulence factors under different growth conditions and antibiotic suscept bility pattern of Aeromonas hydrophila were investigated in this s udy. The virulence actors tested on the isolates included haemolytic activity, exopolysaccharide (capsule) and toxin production. Other cell property evaluated was antibiotic resistance. Of the several chemotherapeutants tested, streptomycin had Minimal Inhibitory Concentration (MIC) and Minimal Cidal Concentration (MCC) at 25 µg/ml; flumequine, MIC 15 µg/ml and MCC 20 µg/ml; nitrofurantoin, MIC 20 µg/ml and MCC at 20 µg/ml; chloramphenicol, MIC 15 µg/ml and MCC 30 µg/ml and nalidixic acid, MIC 25 µg/ml, MCC 30 µg/ml, respectively. Virulence characteristics were apparent from the study; the properties exhibited such as ß-haemolytic activity (F= 1.32551; p < 0.001) and toxin product on (F = 0.141; p > 0.05) were evidence of the pathogenic potential of A. hydrophila.

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