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A New and Simple Method of Confirmatory Detection of Mating in Albino Rats (Rattus Norvegicus)

By Animal Research International, University Of Nigeria, Nsukka.

Summary

Laboratory animal experimentation is an important tool for the investigation and understanding of various biological principles and study of human and animal disease mechanisms.
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Abstract

ABSTRACT A new and simple method o detecting mated female albino rats was developed and tested for precision and accuracy. The method involved the gross observation of grey to yellow sh protein coagulates (remnants of the copulatory plug) on vaginal smears of mated females made on clean glass slides. Results o test of the new method showed that the mean length of time between observation of the protein coagulates on vaginal smears and delivery o the mated pregnant emales was 21.39 ± 0.11 days (range = 20 – 23 days, n = 58), wh ch concurred with the normal 21-day gestation length o rats. The coefficient o variation (CV) of imprecision of the new method was found to be 3.74 %. This new method is simple, easy to apply and does not interfere with fertilization and pregnancy, and also does not involve either the use of specially designed rat cages or microscopy of vaginal smears, which were the constraints of the former methods of confirming mating in rats.

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